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KMID : 1007520090180020519
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Food Science and Biotechnology
2009 Volume.18 No. 2 p.519 ~ p.525
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tructural Characterization of Non-reducing Oligosaccharide Produced by Arthrobacter crystallopoietes N-08
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Bae Bum-Sun
Shin Kwang-Soon
Lee Ho
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Abstract
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A bacterial strain (Strain N-08) capable of extracellularly producing high level of non-reducing oligosaccharide(NR-OS) isolated from soil. The strain was identified phylogenetically by 16S rDNA sequence analysis and found to be veryclose to Arthrobacter crystallopoietes. The high production of NR-OS was observed in the basal culture medium containingmaltose as a sole carbon source. The NR-OS in culture supernatant was purified by glucoamylase treatment and Dowex-1(OH?) ion exchange chromatography and its structure was characterized. This oligosaccharide consisted of only glucose.Methylation analysis indicated that this fraction was composed mainly of non-reducing terminal glucopyranoside. Matrix-assisted laser-induced/ionization time-of-flight (MALDI-TOF) and electrospray ionization-mass spectrometry (ESI-MS)/MSanalysessuggested that this oligosaccharide comprised non-reducing disaccharide unit with 1,1-glucosidic linkage. When thisdisaccharide was analyzed by 1H-NMR and 13C-NMR, it gave the same signals with α-D-glucopyranosyl-(1,1)-α-D-glucopyranoside. These results indicated that the NR-OS produced by A. crystallopoietes N-08 was α1,α1-trehalose. This isthe first report of the trehalose which can be produced directly from maltose by A. crystallopoietes N-08.
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KEYWORD
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structure, non-reducing oligosaccharide, trehalose, Arthrobacter crystallopoietes
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